Torfeno Branishte Nature Reserve from Vrah Ushite (‘Ears Peak’); Cherni Vrah in the background. With its annual average wind speed of 9.3 m/s Cherni Vrah is the second most windy peak in Bulgaria after Peak in the (10.3 m/s).

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The legend holds it that Cherni Vrah was climbed as early as 181 B.C. Nowadays, the easiest access to the peak is by a 1.4-km track from the upper station of Romanski chair lift situated on Peak (2191 m). Cherni Vrah has a special place in. Thanks to its proximity to the peak is by far the most visited mountain summit in the country, attracting tens of thousands of visitors annually. The first group of tourists to climb the peak involved 300 people, among them the contemporary doyen of, and was led by another famous Bulgarian,. The event took place on August 27, 1895, and annual tourist rallies are held on Cherni Vrah on that date considered the birthday of Bulgarian tourist movement.

The of the name Cherni Vrah (Black Peak) is uncertain. Popularly, it is attributed to the black colour of the conspicuous rock formations dominating the peak's tip. Another belief holds it that the name derived from the darker appearance the peak presumably had in the past, when the zone of used to be overgrown by ( Pinus mugo) that was burnt in the to expand. Nowadays only few isolated communities have survived in the vicinity of Cherni Vrah, and their process of expansion and recovery is very slow. Down the ski run on Cherni Vrah's northern slope, with Malak Rezen Peak in the background; Murgash Peak, Balkan Range on the horizon. Climate [ ] Subarctic climate, with an average annual temperature of 0,3C. For the period 1931-1970 the lowest recorded temperature was -27,4C, while the highest - +22,7C.

• Scrape cells off the plate and transfer to microcentrifuge tubes. If necessary, lysate can be stored at -80°C. The supernatant is the cell lysate. • Sonicate on ice three times for 5 sec each. Akt vipolnennih rabot ks2 blank kazahstan. • Microcentrifuge for 10 min at 4°C, 14,000 x g and transfer the supernatant to a new tube.

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